Sequential Injection Chromatography (SIC)is similar to sequential injection , based on flow programming (Chapter 5). In this way Liquid Chromatography (LC) benefits from the versatility of sample processing inherent in SIA. Sample and eluent solutions are sequentially aspirated into the instrument by flow reversal and injected in preprogrammed sequence on the separation column (Satinsky, Solich, Chocholous & Karlicek). Reverse phase, ion-exchange, or affinity chromatography have been successfully carried in SIC mode.
The instrument, comprises a syringe pump, a multiposition valve a separation column and a flow trough detector. Flow programming allows selection of injected sample volume, facilitates precolumn derivatization, generates elution gradients, and speeds up column regeneration. Flow programming also accelerates the assay by fast column flushing. Sichrom instrument is produced byFIAlab.
Šatínský D., Solich P., Chocholouš P., Karlícek R.: Anal. Chim. Acta, 499 (2003) 205-214.