In addition to the protocols outlined in the previous section, many other assay sequences can be envisioned. This is because the structure of any sequence comprises three components: the number of reagents used, the number of transfers between holding coils, and the method used, that is SHC or SFC.
At this early stage of method development it is practical to consider only few basic sequences and to examine their feasibility and potential applications.
Single reagent protocol can be designed without transfer (A), and with single transfer (B)
Feasibility and protocol optimization for single reagent assays is discussed and experimentally verified in (1.2.28.) and used for assay of