The amazing variety of manifolds described in the literature documents versatility of Flow Injection and ingenuity of researchers and practitioners of this technique. Since it is, obviously out of scope of this Tutorial to review even a fraction of this topic, this section is focused on the most frequently used method and applications..
Reagent based assays, (A to E) with spectrophotometry, fluorescence or chemiluminiscence detection are the largest group of cFI methods in use.
Sorbent extraction (F) in cFI format is used to preconcentration target analyte and to remove interfering matrix component. It gained wide acceptance and became useful tool in oceanography and environmental research, replacing previously used solvent extraction (G). Gas diffusion (H) has a niche position for selective separation and assays of volatile compounds. Hansen’s Bibliography and recently published review (Trojanowitcz and Kolacinzka 2016) provide a comprehensive view of CFI methodology.
Applications of cFI have been summarized in several monographs: