Simultaneous Chromatography and Bead Injection Spectrophotometry
Human  IgG Captured on and Eluted from Protein G Sepharose Beads

Sample: 20 ÁL of 0.2 mg/mL Human IgG, Column: Protein G Sepharose 4B. Two elutions. Eluant: 20 ÁL of 0.1 M HCl in PBS, pH = 1.3 Path length: 1.2 mm (on-column), 10 mm (post-column) Flow rate = 1 ÁL /s. Note that the IgG has been completely eluted by the first zone of eluant from the column.